Streptavidin Magnetic Beads

EmerTher Streptavidin Magnetic Beads are nano-superparamagnetic beads covalently coated with highly purified streptavidin. The beads provide a rapid and efficient method to  capture biotinylated molecules, including  DNA,  RNA,  PCR products, oligonucle- otides, antibodies, peptides, and other proteins, which can be subsequently used in a wide variety of applications, including immunopurification, immunoassays, cell isolation, and organelle isolation, etc.

Product Features
  • Magnetic Separation – Fast response, complete purification process within 30 min, no chromatograph, no centrifugation, maximally preserve protein activity

  • Excellent Suspension Ability  – Nanometer scale, rapid conversion from solid-phase to solution and vice versa

  • High Binding Capacity – efficient coupling, higher binding surface area

  • Low non-specific Binding –hydrophilic microspheres, low non-specific binding

  • Fully compatible with automation  and LC-MS/MS analyses

Extraction Procedures

Manual Extraction

Streptavidin Magnetic Beads

 

Biotinylated molecules/cells

 

Impurity

Automated Extraction

The beads are simply added to samples and biotinylated molecules/cells will bind to the beads.

 

After washing unbound impurity off, the biotinylated molecules/cells can be eluted from the magnetic beads or the bound magnetic beads can be directly used in downstream experiments.

 

The process can be completed manually or fully automated for high throughput applications.

Experimental Results

LC-MS/MS quantitative analysis of purified antibody-drug conjugate. Antibody was labelled with biotin, and then bound to EmerTher streptavidin magnetic beads via streptavidin-biotin coupling. Conjugated magnetic beads were subsequently used to purify antibody-drug conjugate from human plasma.

Catalog

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