Ni-NTA (His-tag Affinity) Magnetic Beads
EmerTher Ni-NTA Magnetic Beads are nano-superparamagnetic beads coupled with nickel-charged nitrilotriacetic acid (Ni-NTA). With a fast magnetic response rate, high protein binding capacity and low non-specific binding, EmerTher Ni-NTA Magnetic Beads provide a rapid and efficient method to purify His-tagged fusion proteins from crude cell lysates.
Product Features
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NTA has four chelation sites for nickel ions and thus binds nickel more tightly than other metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel ion leaching and results in a greater binding capacity and higher purity of the extracted protein than those obtained using other metal-chelating purification systems.
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Can be re-used after bead regeneration
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Binding capacity: 10-60 µg His-tagged fusion proteins per mg beads
Extraction Procedures
Manual Extraction
Automated Extraction
Ni-NTA Magnetic Beads
His-tagged fusion Proteins
Impurity
The beads are simply added to a sample and His-tagged fusion proteins will bind to the beads.
After washing unbound impurity off, the proteins can be eluted from the magnetic beads or the bound magnetic beads can be directly used in downstream experiments.
The process can be completed manually or fully automated for high throughput applications.
Experimental Results
AvanBio Ni-NTA magnetic bead purification result
M: marker
1: Purified 6His-fusion protein
2: Bacteria lysate