NHS Magnetic Beads
EmerTher NHS Magnetic Beads are nano-superparamagnetic beads coated with NHS (N-hydroxysuccinimide) functional groups, which react with primary amines on nucleic acid, protein, antibodies or other molecules to form stable amide linkages. After coupling, the beads can be separated from the solution using a magnet for downstream experiments, such as enzymatic reactions, immunoprecipitation of large molecules, cell isolation, and organelle isolation, etc.
Product Features
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Easy to perform: Covalent binding is easily achieved by incubating large molecules and NHS magnetic beads together
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No activation process is needed
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Fast magnetic response
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High binding affinity
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Low non-specific binding
Mechanism
Extraction Procedures
Manual Extraction
NHS Magnetic Beads
Targeted Molecules
Impurity
Automated Extraction
The beads are simply added to a sample and targeted molecules will bind to the beads.
After washing unbound impurity off, the molecules can be eluted from the magnetic beads or the bound magnetic beads can be directly used in downstream experiments.
The process can be completed manually or fully automated for high throughput applications.
Experimental Results
EmerTher NHS magnetic beads and brand P NHS magnetic beads were compared by conjugating with the same enzyme to purify Quercitrin. 25 µg of beads were used for each experiment. Results showed that EmerTher beads extracted five times more proteins than the brand P beads.