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Magnetic Viral RNA Extraction Kit

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EmerTher Magnetic Viral RNA Extraction Kit is used to extract viral RNA from blood, plasma, serum, throat swabs, urine, etc. Purified viral RNA can be used for diagnosis or monitoring of various infectious diseases, such as influenza A and B, avian influenza, hand-foot-and-mouth disease, HCV, HIV, etc. The extraction process is fast, easy, and can be performed entirely at room temperature.


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Product Features

  • High throughput: 12-20 min viral RNA extraction

  • Eco-friendly: Does not contain hazardous organic solvents such as phenol or chloroform, thus overcoming safety issues related to the Trizol method

  • Keep RNA stable: Prevent degradation of RNA during extraction  

  • Automation: Designed to be fully compatible with automation

Extraction Procedures

Manual Extraction

Magnetic beads

DNA

Proteins and other cellular debris

1. Add sample to a lysis-extraction solution to enable cell lysis, RNA release and RNA binding to magnetic beads in one step

2. Apply magnetic force enabling easy wash of the beads with Washing Solution I, II, III

3. Elution of RNA from the beads using water or TE buffer

Automated Extraction

Pre-packed plates for easy automation; compatible with a variety of automatic magnetic bead processors and liquid handling systems

Format 96A

Format 96B

Experimental Results

Hepatitis C virus (HCV) RNA, extracted from HCV-positive serum samples, was measured by fluorescent quantitative PCR

  • Extracted RNA displayed excellent HCV amplification curves

  • Excellent reproducibility of internal standard amplification curves

  • Extremely sensitive virus titer detection:  < 10 IU/ml

Catalog
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