Magnetic FFPE Tissue RNA Extraction Kit
EmerTher Magnetic FFPE Tissue RNA Extraction Kit is used for extracting RNA from formaldehyde fixed- paraffin embedded (FFPE) tissues. Purified RNA can be directly applied for a variety of downstream experiments, including PCR, gene sequencing, etc.
The kit contains superparamagnetic nanoparticles which are efficiently bound to nucleic acids and uses a safe and eco-friendly extraction system. The magnetic beads are coated through a unique process, enabling strong binding with nucleic acid.
Product Features
-
Eliminate the use of toxic solvents, such as xylene, chloroform and phenol
-
Effectively remove cross-linking between nucleic acids and proteins formed during formalin fixation, preventing inhibition of reverse transcription of the extracted RNA due to cross-linkage and increasing the sensitivity and consistency of subsequent tests
-
Fast: Complete RNA extraction within 1hr
-
Can be used with EmerTher magnetic FFPE tissue DNA extraction kit to extract DNA and RNA from one sample simultaneously
Extraction Procedures
Manual Extraction
Magnetic beads
RNA
Proteins and other cellular debris
1. Add sample to a lysis-extraction solution to enable cell lysis, RNA release and RNA binding to magnetic beads in one step
2. Apply magnetic force enabling easy wash of the beads with Washing Solution I, II, III
3. Elution of RNA from the beads using water or TE buffer
Automated Extraction
Pre-packed plates for easy automation; compatible with a variety of automatic magnetic bead processors and liquid handling systems
Format 96B
Experimental Results
Electrophoresis of RNA extracted from FFPE Tissue samples
1-4: FFPE tissue samples from different patients 5: MW marker
Application
Detection of EGFR Expression by Q PCR
-
Easy and fast extraction of RNA from FFPE tissues with high yield and purity
-
Simultaneous extraction of DNA and RNA (Please see the page for the FFPE Tissue DNA Extraction Kit)
Catalog
