Magnetic PCR Product Purification Kit
EmerTher Magnetic PCR Product Purification Kit is intended for fast and simple purification of PCR products (> 100 bp). DNA fragments from PCR reactions selectively bind to beads so that PCR by-products are removed during washing steps. Highly purified DNA is then eluted and can be used directly for downstream applications, including sequencing , restriction digestion, in vitro transcription, and other subsequent experiments.
Product Features
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Completely removes primers and dimers to avoid the interference on sequencing
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Complete removal of enzymes and dNTPs
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High recovery of PCR products (> 100 bp): 75-95%
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No centrifugation or filtration; therefore, the whole procedure can be easily automated
Extraction Procedures
Manual Extraction
Magnetic beads
DNA
primers, dimers, enzymes, unincorporated dNTPs, etc.
1. Mix sample with magnetic beads and PCR products bound to beads
2. Apply magnetic force enabling easy wash of the beads with Wash Solution
3. Elution of DNA from the beads using water or TE buffer
Automated Extraction
Experimental Results
1. DNA ladder
2. PCR product
3-6. Purified PCR product using different batches of EmerTher PCR Product Purification Kits
Generuler DNA ladder purification result: Completely removal of the 50 bp band
Catalog
