Magnetic Circulating Free DNA Extraction Kit

EmerTher Magnetic Circulating Free DNA Extraction Kit is used for extracting circulating free DNA (cfDNA) from acellular tissue fluid such as plasma with high efficiency and is an ideal sample preparation kit for Noninvasive Prenatal Testing (NIPT) or liquid biopsy.

Product Features
Product Features
  • High throughput: Fast extraction, completed in about 1 hr

  • Process 200 µl -3 ml plasma/prep. Sample volume is scalable

  • cfDNA peaks reside at ~170-200 bp with high purity and high concentrations

  • Automation: Compatible with a variety of automatic magnetic bead processors

Extraction Procedures

Manual Extraction

Magnetic beads

DNA

Proteins and other cellular debris

1. Add sample to a lysis-extraction solution to enable cell lysis, DNA release and DNA binding to magnetic beads in one step

3. Elution of DNA from the beads using water or TE buffer

2. Apply magnetic force enabling easy wash of the beads with Washing Solution I & II

Automated Extraction

Pre-packed plates for easy automation; compatible with a variety of automatic magnetic bead processors and liquid handling systems

Format 96A

Format 96B

Format 96D

Experimental Results

Genomic DNA Electrophoresis after DNA Extraction by AvanBio Kit

Genomic DNA Absorption Curve

cfDNA extracted from 23 blood samples (5 cancer patients and 18 healthy volunteers, 1 ml/sample) with EmerTher cfDNA extraction kit and quantified by AccuBlueTM DNA quantitation

Distribution of cfDNA fragment lengths analyzed by PE Labchip (11 plasma samples obtained from volunteers)

The peaks of cfDNA fragments reside at ~170-200 bp with high purity and high concentrations

Representative figures showing distribution of purified cfDNA fragments

Catalog

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Tel:+1-800-868-0176 

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