Magnetic Circulating Free DNA Extraction Kit
EmerTher Magnetic Circulating Free DNA Extraction Kit is used for extracting circulating free DNA (cfDNA) from acellular tissue fluid such as plasma with high efficiency and is an ideal sample preparation kit for Noninvasive Prenatal Testing (NIPT) or liquid biopsy.
Product Features
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High throughput: Fast extraction, completed in about 1 hr
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Process 200 µl -3 ml plasma/prep. Sample volume is scalable
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cfDNA peaks reside at ~170-200 bp with high purity and high concentrations
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Automation: Compatible with a variety of automatic magnetic bead processors
Extraction Procedures
Manual Extraction
Magnetic beads
DNA
Proteins and other cellular debris
1. Add sample to a lysis-extraction solution to enable cell lysis, DNA release and DNA binding to magnetic beads in one step
2. Apply magnetic force enabling easy wash of the beads with Washing Solution I & II
3. Elution of DNA from the beads using water or TE buffer
Automated Extraction
Pre-packed plates for easy automation; compatible with a variety of automatic magnetic bead processors and liquid handling systems
Format 96A
Format 96B
Format 96D
Experimental Results
Genomic DNA Electrophoresis after DNA Extraction by AvanBio Kit
Genomic DNA Absorption Curve
cfDNA extracted from 23 blood samples (5 cancer patients and 18 healthy volunteers, 1 ml/sample) with EmerTher cfDNA extraction kit and quantified by AccuBlueTM DNA quantitation
Distribution of cfDNA fragment lengths analyzed by PE Labchip (11 plasma samples obtained from volunteers)
The peaks of cfDNA fragments reside at ~170-200 bp with high purity and high concentrations
Representative figures showing distribution of purified cfDNA fragments
Catalog
