Chitin (CBD-tag Affinity) Magnetic Beads
AvanBio Chitin Magnetic Beads are nano-superparamagnetic beads covalently coupled with chitin.
With a fast magnetic response rate, high protein binding capacity and low non-specific binding, AvanBio Chitin Magnetic Beads provide a rapid and efficient method to purify CBD(chitin-binding domain)-fusion proteins from cell culture supernatant.
Simple and Efficient Extraction Procedure
The beads are simply added to cell culture supernatant and CBD-fusion proteins will bind to the beads.
After washing unbound proteins off, the CBD-fusion proteins can be eluted from the magnetic beads or the protein-bound magnetic beads can be directly used in downstream experiments (e.g. capturing target proteins, which bind to the immobilized CBD-fusion proteins, from crude cell lysates).
The process can be completed manually or fully automated for high throughput applications.
Magnetic Separation – Fast response, complete purification process within 30 min, no chromatograph, no centrifugation, maximally preserve protein activity
Excellent Suspension Ability – Nanometer scale, rapid conversion from solid-phase to solution and vice versa
High Binding Capacity – efficient coupling, higher binding surface area
Low non-specific Binding –hydrophilic microspheres, low non-specific binding
Fully compatible with automation and LC-MS/MS analyses
pH stability：pH 3-13
30 min sedimentation rate：<0.1%
Magnetic response rate: >30emu/g
Binding capacity: 60-100µg CBD-fusion proteins per mg magnetic beads
1: MW marker
2: Cell lysate
3: Purified CBD-fusion protein (Competitor beads)
4: Purified CBD-fusion protein (AvanBio beads)
Stored at 2-8 °C, 2 years