Amylose (MBP-tag Affinity) Magnetic Beads

AvanBio Amylose Magnetic Beads are nano-superparamagnetic beads covalently coupled with amylose.

 

With a fast magnetic response rate, high protein binding capacity and low non-specific binding, AvanBio Amylose Magnetic Beads provide a rapid and efficient method to purify MBP (maltose binding protein)-fusion proteins from cell culture supernatant.

 

Simple and Efficient Extraction Procedure

The beads are simply added to cell culture supernatant and MBP-fusion proteins will bind to the beads.

 

After washing unbound proteins off, the MBP-fusion proteins can be eluted from the magnetic beads or the protein-bound magnetic beads can be directly used in downstream experiments (e.g. capturing target proteins, which bind to the immobilized MBP-fusion proteins, from crude cell lysates).

 

The process can be completed manually or fully automated for high throughput applications.

Product Features
  • Magnetic Separation – Fast response, complete purification process within 30 min, no chromatograph, no centrifugation, maximally preserve protein activity

  • Excellent Suspension Ability  – Nanometer scale, rapid conversion from solid-phase to solution and vice versa

  • High Binding Capacity – efficient coupling, higher binding surface area

  • Low non-specific Binding –hydrophilic microspheres, low non-specific binding

  • Fully compatible with automation  and LC-MS/MS analyses

Specifications
  • Diameter:500nm

  • pH stability:pH 3-13

  • 30 min sedimentation rate:<0.1%

  • Magnetic response rate: >30emu/g

  • Solvent:20% ethanol

  • Binding capacity:  10-100µg MBP-fusion proteins per mg magnetic beads 

Components
Results

1. Marker

2. Cell Lysis

3. Competitor A Magnetic Beads

4. AvanBio Amylose Magnetic Beads

Storage

Stored at 2-8 °C, 2 years 

Copyright © 2015 by Avanbio Inc. All rights reserved.

Contact Us

239 New Road, Suite A-107
Parsippany, NJ 07054
U.S.A

Tel:+1-800-868-0176 

Email: support@avanbio.com 

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