Amylose (MBP-tag Affinity) Magnetic Beads
AvanBio Amylose Magnetic Beads are nano-superparamagnetic beads covalently coupled with amylose.
With a fast magnetic response rate, high protein binding capacity and low non-specific binding, AvanBio Amylose Magnetic Beads provide a rapid and efficient method to purify MBP (maltose binding protein)-fusion proteins from cell culture supernatant.
Simple and Efficient Extraction Procedure
The beads are simply added to cell culture supernatant and MBP-fusion proteins will bind to the beads.
After washing unbound proteins off, the MBP-fusion proteins can be eluted from the magnetic beads or the protein-bound magnetic beads can be directly used in downstream experiments (e.g. capturing target proteins, which bind to the immobilized MBP-fusion proteins, from crude cell lysates).
The process can be completed manually or fully automated for high throughput applications.
Product Features
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Magnetic Separation – Fast response, complete purification process within 30 min, no chromatograph, no centrifugation, maximally preserve protein activity
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Excellent Suspension Ability – Nanometer scale, rapid conversion from solid-phase to solution and vice versa
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High Binding Capacity – efficient coupling, higher binding surface area
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Low non-specific Binding –hydrophilic microspheres, low non-specific binding
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Fully compatible with automation and LC-MS/MS analyses
Specifications
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Diameter：500nm
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pH stability：pH 3-13
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30 min sedimentation rate：<0.1%
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Magnetic response rate: >30emu/g
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Solvent：20% ethanol
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Binding capacity: 10-100µg MBP-fusion proteins per mg magnetic beads
Components
Results
1. Marker
2. Cell Lysis
3. Competitor A Magnetic Beads
4. AvanBio Amylose Magnetic Beads
Storage
Stored at 2-8 °C, 2 years